Examination of eight cases of multiple sclerosis and 56 neurological and non-neurological controls for genomic sequences of measles virus, canine distemper virus, simian virus 5 and rubella virus.

Molecular and Immunological Investigation of Canine Distemper Virus (CDV) and Its Co- Infection with Canine Parainfluenza Virus Type 2

Background and Objective: Canine distemper (CD) is a highly transmissible serious disease of carnivores. Distemper virus has immunosuppression effects, which, in turn, could lead to opportunistic infections. The present study was performed to detect CDV by the genomic and immunological methods and investigate its co- infection with canine parainfluenza virus type 2 (CPiV-2). Materials and Meth...

Aphylogenetic study on the NP gene of detected canine distemper virus in (2008-2011) Iran

BACKGROUND: Canine Distemper (CD) is a lethal systemicdisease affecting a wide variety of terrestrial carnivores.OBJECTIVES: This study was performed to survey epidemiologicaland molecular characteristics of the canine distemper virus(CDV) strains circulating in Iran. METHODS: In this study, 19CDV-suspected unvaccinated dogs from Northeast and center ofIran were analyzed for presence of CDV nuc...

Use of human diploid cell MRC - 5, for production of Measles and Rubella virus vaccines

Construction of a Minigenome Rescue System for Measles Virus, AIK-c Strain

Background:In the recent decade, the reverse genetics method has been broadly used for rescue of negative-stranded RNA viruses from cDNA or viral minigenomes. This technique has been applied to study different steps in virus replication and virus-host interactions. Reverse genetics could also be implemented for design of new vaccines. The T7 RNA polymerase activity as well as virus (nucleocapsi...

Detection and differentiation of wild-type and vaccine strains of canine distemper virus by a duplex reverse transcription polymerase chain reaction

Canine distemper virus (CDV) is the cause of canine distemper (CD) which is a severe and highly contagious disease in dogs. In the present study, a duplex reverse transcription polymerase chain reaction (RT-PCR) method was developed for the detection and differentiation of wild-type and vaccine strains of CDV. Four primers were designed to detect and discriminate the two viruses by generating 6...